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Biology · Transport in Cells

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Diffusion

Cells constantly need to take things in and get rid of waste. Much of this happens by diffusion, with no energy needed. This lesson explains what diffusion is, which substances move this way, and what makes it faster or slower.

  • 9 key terms
  • All boards

Learning Objectives

  1. 1Define diffusion in terms of particles, concentration and net movement.
  2. 2Give examples of substances that move into and out of cells by diffusion.
  3. 3Explain how the concentration gradient, the temperature and the surface area of the membrane affect the rate of diffusion.
  4. 4Calculate a rate of diffusion from a change and the time taken.

Retrieval practice

  1. 1

    What does the cell membrane do?

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    It controls which substances pass into and out of the cell.

  2. 2

    Which sub-cellular structure releases energy by aerobic respiration?

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    The mitochondrion.

  3. 3

    What is meant by a concentrated solution?

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    One with a lot of dissolved substance in it compared with the amount of water.

  4. 4

    Name the process that makes two genetically identical cells from one.

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    Mitosis.

Movement without energy

Every cell takes in oxygen and food and gets rid of carbon dioxide and other waste, and all of it has to cross the cell membrane. Many substances do this by diffusion. It needs no energy from the cell, because the particles move on their own. That makes diffusion the cheapest way for a cell to move things, and the reason it matters so much in your body.

What is diffusion?

Diffusion is the spreading out of the particles of any substance in solution, or particles of a gas, resulting in a net movement from an area of higher concentration to an area of lower concentration.

  • Particles are always moving

    The particles of a gas, or of a substance dissolved in a liquid, move about randomly all the time.

  • Net movement

    Particles move in every direction, but there are more particles in the region of higher concentration, so more of them move out of it than into it.

  • Down the concentration gradient

    The difference in concentration between two places is the concentration gradient. Particles move down it, from high to low.

  • No energy needed

    The particles use their own movement, so the cell does not have to use energy from respiration.

Substances that diffuse in living things

Some of the substances that move into and out of cells by diffusion are oxygen and carbon dioxide in gas exchange, and the waste product urea.

  • Oxygen

    Cells use oxygen for respiration, so it is always at a lower concentration inside a cell than outside. Oxygen diffuses in.

  • Carbon dioxide

    Respiration makes carbon dioxide, so it is at a higher concentration inside the cell than outside. It diffuses out.

  • Urea

    Urea is a waste product made in cells. It diffuses from the cells into the blood plasma, and the kidneys then remove it from the blood so that it can be excreted.

Following oxygen from the air to a muscle cell

Diffusion happens at each stage, because each place has a lower concentration of oxygen than the one before.

  1. 1 Breathing in

    Air in the lungs has a high concentration of oxygen.

  2. 2 Into the blood

    Oxygen diffuses from the air in the alveoli into the blood, which has a lower concentration of oxygen.

  3. 3 Round the body

    The blood carries the oxygen to the muscles.

  4. 4 Into the cells

    The muscle cells use oxygen up, so its concentration in them is low, and oxygen diffuses from the blood into them.

Three things that change the rate of diffusion

The rate of diffusion is how fast the particles spread. Three factors affect it.

  • Concentration gradient

    The bigger the difference in concentration, the faster the diffusion. Many more particles move one way than the other.

  • Temperature

    At a higher temperature the particles have more energy and move faster, so they spread out faster.

  • Surface area of the membrane

    The larger the area of membrane, the more particles can cross it at once, so the faster the diffusion.

Calculating a rate of diffusion

A cell takes in 24 micrograms of a substance by diffusion in 8 minutes. Calculate the mean rate of uptake in micrograms per minute.

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  1. 1 Write the rate as a change divided by a time Rate = amount taken in ÷ time taken.
  2. 2 Put in the numbers Rate = 24 ÷ 8.
  3. 3 Work it out, and give the unit Rate = 3 micrograms per minute.

Answer3 micrograms per minute.

Case study

Gas exchange in the air sacs of the lungs

Air that is breathed in has about 21% oxygen and air that is breathed out has about 16%. Oxygen diffuses from the air in the alveoli into the blood, and carbon dioxide diffuses the other way. The gradient stays steep because breathing keeps bringing fresh air in and the flowing blood keeps carrying oxygen away.

21% Oxygen in the air breathed in (approximately)
16% Oxygen in the air breathed out (approximately)

The definition to learn

Diffusion is the net movement of particles from an area of higher concentration to an area of lower concentration.

Say "net movement": particles move in both directions, but more go one way than the other.

Diffusion

Diffusion

  • What it is

    • net movement of particles from high to low concentration
  • Examples

    • oxygen
    • carbon dioxide
    • urea
  • No energy

    • the particles move on their own
  • Concentration gradient

    • steeper gradient
    • faster diffusion
  • Temperature

    • warmer
    • faster
  • Surface area

    • larger area
    • faster

Summary and exam focus

  • Diffusion is the spreading out of particles, resulting in a net movement from a higher to a lower concentration.
  • Oxygen, carbon dioxide and urea move into and out of cells by diffusion.
  • A bigger concentration difference makes diffusion faster.
  • A higher temperature makes diffusion faster.
  • A larger surface area of membrane makes diffusion faster.
  • Diffusion does not need energy from respiration.

Exam focus

Explain three factors that affect the rate of diffusion. (6 marks) (6 marks)

For each factor, give the change and the effect together: "the greater the concentration difference, the faster the diffusion". For temperature, say that the particles have more energy and move faster. Do not say that particles "want" to spread out.

Key terms

The words this lesson expects you to use. Each one is linked from the first place it appears above.

Diffusion
The spreading out of particles of a gas, or of a substance in solution, resulting in a net movement from an area of higher concentration to an area of lower concentration.
Concentration
The amount of a substance in a given volume.
Concentration gradient
The difference in concentration between two places.
Net movement
The overall movement of particles in one direction, after those moving the other way are taken into account.
Particle
A very small piece of a substance, such as a molecule of oxygen.
Cell membrane
The thin layer around a cell that controls what enters and leaves it.
Urea
A waste product made in cells, which diffuses into the blood plasma and is excreted by the kidneys.
Rate
How much something changes in a given time.
Surface area
The total area of the outside of an object, or of a membrane.

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